What is the catalog number for NEFL ELISA Kit (Rat)?

The catalog number for NEFL ELISA Kit (Rat) is OKCA02580-96W.

What sizes are available for NEFL ELISA Kit (Rat)?

NEFL ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is NEFL ELISA Kit (Rat) in stock?

NEFL ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

NEFL ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay: CV

CAS Number

7732-18-5

Gene Aliases

Nfl, NF-L, NF68

Gene ID

83613

Accession Number

NP_113971

Reactivity

Rat

Target

Neurofilaments usually contain three intermediate filament proteins: L, M, and H which are involved in the maintenance of neuronal caliber.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Nefl ELISA Kit (Rat) (OKCA02580) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Nefl has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Nefl is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Nefl captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

7.8 - 500 pg/mL

Recovery

Sample Type Mean Recovery (%) Range (%) Serum (n=5) 90 82-94 EDTA Plasma (n=4) 99 91-104 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 90 82-94 Serum (n=5) 90 82-94 EDTA Plasma (n=4) 99 91-104 EDTA Plasma (n=4) 99 91-104