SERPINH1 ELISA Kit (Rat)
Selected Variant
OKCA02556-96W
Size: 96 Well
Product Specifications
Reproducibility
Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Aliases
Cbp2, Serpinh2
Gene ID
29345
Accession Number
NP_058869
Reactivity
Rat
Target
Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Serpinh1 ELISA Kit (Rat) (OKCA02556) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpinh1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Serpinh1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Serpinh1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates
Detection Range
0.156 - 10 ng/mL
Recovery
Sample Type Mean Recovery (%) Range (%) Serum (n=5) 92 89-96 EDTA Plasma (n=4) 87 83-92 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 92 89-96 Serum (n=5) 92 89-96 EDTA Plasma (n=4) 87 83-92 EDTA Plasma (n=4) 87 83-92
Sensitivity
0.039 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
SERPINH1
Protein Name
Serpin H1
Gene Name URL
SERPINH1
Nucleotide Accession Number
NM_017173
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