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SERPINH1 ELISA Kit (Rat)

Selected Variant

OKCA02556-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay: CV

CAS Number

7732-18-5

Gene Aliases

Cbp2, Serpinh2

Gene ID

29345

Accession Number

NP_058869

Reactivity

Rat

Target

Binds specifically to collagen. Could be involved as a chaperone in the biosynthetic pathway of collagen.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Serpinh1 ELISA Kit (Rat) (OKCA02556) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Serpinh1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Serpinh1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Serpinh1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Lysates

Detection Range

0.156 - 10 ng/mL

Recovery

Sample Type Mean Recovery (%) Range (%) Serum (n=5) 92 89-96 EDTA Plasma (n=4) 87 83-92 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 92 89-96 Serum (n=5) 92 89-96 EDTA Plasma (n=4) 87 83-92 EDTA Plasma (n=4) 87 83-92

Sensitivity

0.039 ng/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

SERPINH1

Protein Name

Serpin H1

Gene Name URL

SERPINH1

Nucleotide Accession Number

NM_017173

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