APOM ELISA Kit (Rat)
Product Specifications
Reproducibility
Mean Inter-Assay: CV
Gene Aliases
DADB-127H9.5, G3a, HSPC336, MGC22400, NG20, NG20-like protein, alternative name: G3a, NG20
Gene ID
55939
Accession Number
NP_062246
Reactivity
Rat
Target
Probably involved in lipid transport. Can bind sphingosine-1-phosphate, myristic acid, palmitic acid and stearic acid, retinol, all-trans-retinoic acid and 9-cis-retinoic acid.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Apom ELISA Kit (Rat) (OKCA02528) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Apom has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Apom is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Apom captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
1.56 - 100 ng/mL
Recovery
Sample Type Mean Recovery (%) Range (%) Serum (n=5) 91 87-94 EDTA Plasma (n=4) 95 90-99 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 91 87-94 Serum (n=5) 91 87-94 EDTA Plasma (n=4) 95 90-99 EDTA Plasma (n=4) 95 90-99
Sensitivity
0.39 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
APOM
Protein Name
Apolipoprotein M
Gene Name URL
APOM
CAS Number
7732-18-5
Nucleotide Accession Number
NM_019373
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