S100A4 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay: CV

CAS Number

7732-18-5

Gene Aliases

C, FS, pk, 18A, 42a, Mts, PeL, cal, 18A2, Capl, FSp1, Mts1, meta, pk9a, PeL98, metastasin

Gene ID

20198

Accession Number

NP_035441

Reactivity

Mouse

Type

ELISA Kit

Assay Principle

Aviva Systems Biology S100a4 ELISA Kit (Mouse) (OKCA02438) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for S100a4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for S100a4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard S100a4 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Culture Supernates, Tissue Homogenates

Detection Range

62.5 - 4,000 pg/mL

Recovery

Sample Type Mean Recovery (%) Range (%) Serum (n=5) 91 85-96 EDTA Plasma (n=4) 94 90-98 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 91 85-96 Serum (n=5) 91 85-96 EDTA Plasma (n=4) 94 90-98 EDTA Plasma (n=4) 94 90-98