LDHA ELISA Kit (Mouse)

Aviva Systems Biology Ldha ELISA Kit (Mouse) (OKCA02404) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with Ldha. Sample or standards are added to the wells along with a fixed quantity of HRP-Conjugated Ldha Detector Antibody and incubated. The Ldha found in the sample or standards competes with immobilized Ldha for binding with the HRP-Conjugated Ldha Detector Antibody. Wells are washed and an enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product. Addition of acidic stop solution changes the coloration to yellow. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Ldhacaptured in the well and inversely proportional to the amount of Ldha which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type Mean Recovery (%) Range (%) Serum (n=5) 101 96-108 EDTA Plasma (n=4) 85 81-90 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 101 96-108 Serum (n=5) 101 96-108 EDTA Plasma (n=4) 85 81-90 EDTA Plasma (n=4) 85 81-90