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GRP ELISA Kit (Mouse)

Selected Variant

OKCA02384-96W

Size: 96 Well

Product Specifications

Reproducibility

Mean Inter-Assay: CV

CAS Number

7732-18-5

Gene Aliases

B, BLP

Gene ID

225642

Accession Number

NP_778177

Reactivity

Mouse

Target

GRP stimulates gastrin release as well as other gastrointestinal hormones. Operates as a negative feedback regulating fear and established a causal relationship between GRP-receptor gene expression, long-term potentiation, and amygdala-dependent memory for fear.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Grp ELISA Kit (Mouse) (OKCA02384) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Grp has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Grp is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Grp captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

39.063 - 2,500 pg/mL

Recovery

Sample Type Mean Recovery (%) Range (%) Serum (n=5) 94 89-98 EDTA Plasma (n=4) 89 82-96 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 94 89-98 Serum (n=5) 94 89-98 EDTA Plasma (n=4) 89 82-96 EDTA Plasma (n=4) 89 82-96

Sensitivity

9.77 pg/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

GRP

Protein Name

Gastrin-releasing peptide

Gene Name URL

GRP

Nucleotide Accession Number

NM_175012

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