What is the catalog number for CFI ELISA Kit (Mouse)?

The catalog number for CFI ELISA Kit (Mouse) is OKCA02361-96W.

What sizes are available for CFI ELISA Kit (Mouse)?

CFI ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is CFI ELISA Kit (Mouse) in stock?

CFI ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

CFI ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay: CV

CAS Number

7732-18-5

Gene Aliases

AHUS3, C3BINA, C3b-INA, FI, IF, KAF, C3B/C4B inactivator, C3b-inactivator, Konglutinogen-activating factor, complement component I, complement control protein factor I, complement factor I heavy chain, li

Gene ID

12630

Accession Number

NP_031712

Reactivity

Mouse

Target

Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Cfi ELISA Kit (Mouse) (OKCA02361) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cfi has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cfi is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Cfi captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Lysates

Detection Range

0.78 - 50 ng/mL

Recovery

Sample Type Mean Recovery (%) Range (%) Serum (n=5) 102 98-106 EDTA Plasma (n=4) 85 81-89 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 102 98-106 Serum (n=5) 102 98-106 EDTA Plasma (n=4) 85 81-89 EDTA Plasma (n=4) 85 81-89