CFI ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay: CV
Gene Aliases
AHUS3, C3BINA, C3b-INA, FI, IF, KAF, C3B/C4B inactivator, C3b-inactivator, Konglutinogen-activating factor, complement component I, complement control protein factor I, complement factor I heavy chain, li
Gene ID
12630
Accession Number
NP_031712
Reactivity
Mouse
Target
Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Cfi ELISA Kit (Mouse) (OKCA02361) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cfi has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cfi is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Cfi captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates
Detection Range
0.78 - 50 ng/mL
Recovery
Sample Type Mean Recovery (%) Range (%) Serum (n=5) 102 98-106 EDTA Plasma (n=4) 85 81-89 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 102 98-106 Serum (n=5) 102 98-106 EDTA Plasma (n=4) 85 81-89 EDTA Plasma (n=4) 85 81-89
Sensitivity
0.195 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
CFI
Protein Name
Complement factor I
Gene Name URL
CFI
CAS Number
7732-18-5
Nucleotide Accession Number
NM_007686
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