ENHO ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Aliases
A, 1110065P19Rik, 2310040A07Rik
Gene ID
69638
Accession Number
NP_081423
Reactivity
Mouse
Target
Involved in the regulation of glucose homeostasis and lipid metabolism.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Enho ELISA Kit (Mouse) (OKCA02330) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Enho has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Enho is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Enho captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
93.75 - 6,000 pg/mL
Recovery
Sample Type Mean Recovery (%) Range (%) Serum (n=5) 103 92-106 EDTA Plasma (n=4) 99 88-103 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 103 92-106 Serum (n=5) 103 92-106 EDTA Plasma (n=4) 99 88-103 EDTA Plasma (n=4) 99 88-103
Sensitivity
23.44 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
ENHO
Protein Name
Adropin
Gene Name URL
ENHO
Nucleotide Accession Number
NM_027147
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