What is the catalog number for TFF3 ELISA Kit (Human)?

The catalog number for TFF3 ELISA Kit (Human) is OKCA02317-96W.

What sizes are available for TFF3 ELISA Kit (Human)?

TFF3 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is TFF3 ELISA Kit (Human) in stock?

TFF3 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What is the molecular weight of the target protein?

Molecular weight information, where applicable, is listed on the datasheet under target details, useful for confirming the correct band on western blot.

How long will it take to receive this product if it’s custom made?

Lead times depend on the product type: custom polyclonal antibodies typically take 12-14 weeks, monoclonal antibodies 15-17 weeks, made-to-order recombinant proteins 4-8 weeks, and made-to-order recombinant antibodies 6-8 weeks. These are estimates and may shift due to production delays.

What is the recommended dilution for this antibody?

A recommended dilution range for each validated application is provided on the datasheet, but optimal dilution should be titrated for your specific experimental system, sample type, and detection method.

TFF3 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay: CV

CAS Number

7732-18-5

Gene Aliases

ITF, P1B, TFI, trefoil factor 3

Gene ID

517889

Accession Number

NP_001107981

Reactivity

Human

Target

Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen) .

Type

ELISA Kit

Assay Principle

Aviva Systems Biology TFF3 ELISA Kit (Human) (OKCA02317) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for TFF3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for TFF3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard TFF3 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Urine, Tissue Homogenates

Detection Range

1.56 - 100 ng/mL

Recovery

Sample Type Mean Recovery (%) Range (%) Serum (n=5) 95 89-100 EDTA Plasma (n=4) 96 87-102 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 95 89-100 Serum (n=5) 95 89-100 EDTA Plasma (n=4) 96 87-102 EDTA Plasma (n=4) 96 87-102