What is the catalog number for SRD5A1 ELISA Kit (Rat)?

The catalog number for SRD5A1 ELISA Kit (Rat) is OKCA02252-96W.

What sizes are available for SRD5A1 ELISA Kit (Rat)?

SRD5A1 ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is SRD5A1 ELISA Kit (Rat) in stock?

SRD5A1 ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

How do I reconstitute this lyophilized product?

Reconstitution instructions, including the recommended diluent and final concentration, are provided on the datasheet. Aliquot immediately after reconstitution to avoid repeated freeze-thaw cycles.

SRD5A1 ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Variation

CAS Number

7732-18-5

Gene Aliases

S5AR 1

Gene ID

24950

Accession Number

NP_058766

Reactivity

Rat

Target

Converts testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Srd5a1 ELISA Kit (Rat) (OKCA02252) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SRD5A1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SRD5A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SRD5A1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Culture Supernates And Tissue Homogenates

Detection Range

15.6 pg/mL-1,000 pg/mL

Recovery

Sample Type Mean (%) Range (%) Serum (n=5) 101 98-105 EDTA plasma (n=4) 95 90-100 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 101 98-105 Serum (n=5) 101 98-105 EDTA plasma (n=4) 95 90-100 EDTA plasma (n=4) 95 90-100