What is the catalog number for OSM ELISA Kit (Rat)?

The catalog number for OSM ELISA Kit (Rat) is OKCA02245-96W.

What sizes are available for OSM ELISA Kit (Rat)?

OSM ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is OSM ELISA Kit (Rat) in stock?

OSM ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

Can this product be used for diagnostic or therapeutic purposes?

No. This product, like all items we supply, is intended for research laboratory use only and must not be used for diagnostics, therapeutics, treatment, or consumption by humans or animals.

What is the shelf life of this product?

Shelf life and expiration dates vary by product and are shown on the product listing and label. Products used beyond their expiration date are not eligible for performance-related returns or refunds, so please check the lot number and expiry before use.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

OSM ELISA Kit (Rat)

Product Specifications

Reproducibility

Mean Inter-Assay Variation

CAS Number

7732-18-5

Gene ID

289747

Accession Number

NP_001006962

Reactivity

Rat

Target

Growth regulator. Inhibits the proliferation of a number of tumor cell lines. It regulates cytokine production, including IL-6, G-CSF and GM-CSF from endothelial cells.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Osm ELISA Kit (Rat) (OKCA02245) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for OSM has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for OSM is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample OSM captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma

Detection Range

15.6 pg/mL-1,000 pg/mL

Recovery

Sample Type Mean (%) Range (%) Serum (n=5) 96 92-102 EDTA plasma (n=4) 89 82-93 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 96 92-102 Serum (n=5) 96 92-102 EDTA plasma (n=4) 89 82-93 EDTA plasma (n=4) 89 82-93