What is the catalog number for BRAF ELISA Kit (Human)?

The catalog number for BRAF ELISA Kit (Human) is OKCA02146-96W.

What sizes are available for BRAF ELISA Kit (Human)?

BRAF ELISA Kit (Human) is available in the following size(s): 96 Well.

Is BRAF ELISA Kit (Human) in stock?

BRAF ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

What dilution range is recommended for immunohistochemistry (IHC) with this antibody?

A starting IHC dilution range is listed on the datasheet, but should be optimized for your specific tissue type and staining protocol.

Can I request a Safety Data Sheet (SDS) for this product?

Yes, safety data sheets are available for hazardous or chemically classified products and can be provided on request or downloaded from the product page.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

BRAF ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Variation

CAS Number

7732-18-5

Gene Aliases

NS7, B-raf, BRAF1, RAFB1, B-RAF1

Gene ID

673

Accession Number

NP_004324

Reactivity

Human

Target

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus. May play a role in the postsynaptic responses of hippocampal neuron. Phosphorylates MAP2K1, and thereby contributes to the MAP kinase signal transduction pathway.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology BRAF ELISA Kit (Human) (OKCA02146) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BRAF has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BRAF is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample BRAF captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Lysates

Detection Range

31.25 pg/mL-2,000 pg/mL

Recovery

Sample Type Mean (%) Range (%) Serum (n=5) 95 89-104 EDTA plasma (n=4) 97 90-100 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 95 89-104 Serum (n=5) 95 89-104 EDTA plasma (n=4) 97 90-100 EDTA plasma (n=4) 97 90-100