IL22RA2 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-Assay Variation
CAS Number
7732-18-5
Gene Aliases
CRF2X, CRF2-10, CRF2-S1, IL-22BP, IL-22RA2, ZCYTOR16, IL-22R-alpha-2
Gene ID
116379
Accession Number
NP_443194
Reactivity
Human
Target
Isoform 2 is a receptor for IL22. Binds to IL22, prevents interaction with the functional IL-22R complex and blocks the activity of IL22 (in vitro) . May play an important role as an IL22 antagonist in the regulation of inflammatory responses. Isoform 1 may play a role in establishing and maintaining successful pregnancy.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology IL22RA2 ELISA Kit (Human) (OKCA02113) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL22RA2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL22RA2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL22RA2 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
31.25 pg/mL-2,000 pg/mL
Recovery
Sample Type Mean (%) Range (%) Serum (n=5) 102 98-105 EDTA plasma (n=4) 98 93-104 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 102 98-105 Serum (n=5) 102 98-105 EDTA plasma (n=4) 98 93-104 EDTA plasma (n=4) 98 93-104
Sensitivity
7.81 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
IL22RA2
Protein Name
Interleukin-22 receptor subunit alpha-2
Gene Name URL
IL22RA2
Nucleotide Accession Number
NM_052962
Frequently Asked Questions
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