MS4A1 ELISA Kit (Human)
Product Specifications
Reproducibility
Mean Inter-Assay Variation
CAS Number
7732-18-5
Gene Aliases
B1, S7, Bp35, CD20, FMC7, CVID5, MS4A2, LEU-16
Gene ID
931
Accession Number
NP_068769
Reactivity
Human
Target
This protein may be involved in the regulation of B-cell activation and proliferation.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology MS4A1 ELISA Kit (Human) (OKCA02077) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MS4A1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MS4A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MS4A1 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma And Tissue Homogenates
Detection Range
3.12 ng/mL-200 ng/mL
Recovery
Sample Type Mean (%) Range (%) Serum (n=5) 97 92-103 EDTA plasma (n=4) 95 89-99 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 97 92-103 Serum (n=5) 97 92-103 EDTA plasma (n=4) 95 89-99 EDTA plasma (n=4) 95 89-99
Sensitivity
0.78 ng/mL.
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
MS4A1
Protein Name
B-lymphocyte antigen CD20
Gene Name URL
MS4A1
Nucleotide Accession Number
NM_021950
Frequently Asked Questions
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