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MS4A1 ELISA Kit (Human)

Product Specifications

Reproducibility

Mean Inter-Assay Variation

CAS Number

7732-18-5

Gene Aliases

B1, S7, Bp35, CD20, FMC7, CVID5, MS4A2, LEU-16

Gene ID

931

Accession Number

NP_068769

Reactivity

Human

Target

This protein may be involved in the regulation of B-cell activation and proliferation.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology MS4A1 ELISA Kit (Human) (OKCA02077) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MS4A1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MS4A1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MS4A1 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma And Tissue Homogenates

Detection Range

3.12 ng/mL-200 ng/mL

Recovery

Sample Type Mean (%) Range (%) Serum (n=5) 97 92-103 EDTA plasma (n=4) 95 89-99 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 97 92-103 Serum (n=5) 97 92-103 EDTA plasma (n=4) 95 89-99 EDTA plasma (n=4) 95 89-99

Sensitivity

0.78 ng/mL.

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

MS4A1

Protein Name

B-lymphocyte antigen CD20

Gene Name URL

MS4A1

Nucleotide Accession Number

NM_021950

Frequently Asked Questions

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