What is the catalog number for EPO ELISA Kit (Horse | Equine)?

The catalog number for EPO ELISA Kit (Horse | Equine) is OKCA02067-96W.

What sizes are available for EPO ELISA Kit (Horse | Equine)?

EPO ELISA Kit (Horse | Equine) is available in the following size(s): 96 Well.

Is EPO ELISA Kit (Horse | Equine) in stock?

EPO ELISA Kit (Horse | Equine) is currently made to order / not held in immediate stock. Contact us for lead time.

Is there an additional fee for hazardous or dry ice shipping of this product?

Yes, if this product requires dry ice or is classed as hazardous for transport, the additional fee will be explicitly shown in your quotation before you confirm the order, so there are no surprise charges later.

How many freeze-thaw cycles can this product tolerate?

We recommend aliquoting the product upon receipt to avoid repeated freeze-thaw cycles, which can reduce activity and stability. This is your responsibility once the product has been delivered.

Is antigen retrieval required for IHC with this antibody?

Recommended antigen retrieval methods, where applicable, are listed on the datasheet under the IHC protocol section.

EPO ELISA Kit (Horse | Equine)

Product Specifications

Reproducibility

Mean Inter-Assay Variation

CAS Number

7732-18-5

Gene Aliases

Erythropoietin

Gene ID

100033849

Accession Number

NP_001075294

Reactivity

Horse | Equine

Target

Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology EPO ELISA Kit (Horse | Equine) (OKCA02067) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EPO has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EPO is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample EPO captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Lysates

Detection Range

0.156 ng/mL-10 ng/mL

Recovery

Sample Type Mean (%) Range (%) Serum (n=5) 90 85-97 EDTA plasma (n=4) 106 101-113 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 90 85-97 Serum (n=5) 90 85-97 EDTA plasma (n=4) 106 101-113 EDTA plasma (n=4) 106 101-113