What is the catalog number for MMP9 ELISA Kit (Dog)?

The catalog number for MMP9 ELISA Kit (Dog) is OKCA02057-96W.

What sizes are available for MMP9 ELISA Kit (Dog)?

MMP9 ELISA Kit (Dog) is available in the following size(s): 96 Well.

Is MMP9 ELISA Kit (Dog) in stock?

MMP9 ELISA Kit (Dog) is currently made to order / not held in immediate stock. Contact us for lead time.

Can this product be used for co-immunoprecipitation (Co-IP)?

Suitability for Co-IP depends on the specific antibody and is noted under validated applications on the datasheet. If it isn’t listed, contact our technical support team for guidance based on your target and sample type.

Is this kit compatible with automated liquid handling platforms?

Automation compatibility varies by kit and isn’t always listed on the datasheet. Contact our technical support team with details of your platform so we can confirm with the manufacturer.

What applications has this product been validated for?

Validated applications such as WB, ELISA, IHC, ICC, or FC are listed on the product datasheet. If you need application-specific validation data before ordering, contact our technical support team with the catalogue number.

MMP9 ELISA Kit (Dog)

Product Specifications

Reproducibility

Mean Inter-Assay Variation

CAS Number

7732-18-5

Gene ID

403885

Accession Number

NP_001003219

Reactivity

Dog

Target

Could play a role in bone osteoclastic resorption. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology MMP9 ELISA Kit (Dog) (OKCA02057) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MMP9 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MMP9 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MMP9 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma

Detection Range

12.5 ng/mL-800 ng/mL

Recovery

Sample Type Mean (%) Range (%) Serum (n=5) 85 81-89 EDTA plasma (n=4) 97 90-105 Sample Type Mean (%) Range (%) Sample Type Mean (%) Range (%) Serum (n=5) 85 81-89 Serum (n=5) 85 81-89 EDTA plasma (n=4) 97 90-105 EDTA plasma (n=4) 97 90-105