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CD81 ELISA Kit (Mouse)

Product Specifications

Reproducibility

Mean Inter-Assay: CV

CAS Number

7732-18-5

Gene Aliases

Ta, Tap, Tsp, Tapa1, Tapa-1, Tspan28

Gene ID

12520

Accession Number

NP_598416

Reactivity

Mouse

Target

May play an important role in the regulation of lymphoma cell growth. May be involved in the acrosome reaction.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Cd81 ELISA Kit (Mouse) (OKCA02018) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd81 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cd81 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Cd81 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Lysates

Detection Range

46.88 - 3,000 pg/mL

Recovery

Sample Type Mean Recovery (%) Range (%) Serum (n=5) 93 89-98 EDTA Plasma (n=4) 104 100-113 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 93 89-98 Serum (n=5) 93 89-98 EDTA Plasma (n=4) 104 100-113 EDTA Plasma (n=4) 104 100-113

Sensitivity

11.72 pg/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

CD81

Protein Name

CD81 antigen

Gene Name URL

CD81

Nucleotide Accession Number

NM_133655

Frequently Asked Questions

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