CD81 ELISA Kit (Mouse)
Product Specifications
Reproducibility
Mean Inter-Assay: CV
CAS Number
7732-18-5
Gene Aliases
Ta, Tap, Tsp, Tapa1, Tapa-1, Tspan28
Gene ID
12520
Accession Number
NP_598416
Reactivity
Mouse
Target
May play an important role in the regulation of lymphoma cell growth. May be involved in the acrosome reaction.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Cd81 ELISA Kit (Mouse) (OKCA02018) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Cd81 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Cd81 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample or standard Cd81 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates
Detection Range
46.88 - 3,000 pg/mL
Recovery
Sample Type Mean Recovery (%) Range (%) Serum (n=5) 93 89-98 EDTA Plasma (n=4) 104 100-113 Sample Type Mean Recovery (%) Range (%) Sample Type Mean Recovery (%) Range (%) Serum (n=5) 93 89-98 Serum (n=5) 93 89-98 EDTA Plasma (n=4) 104 100-113 EDTA Plasma (n=4) 104 100-113
Sensitivity
11.72 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
CD81
Protein Name
CD81 antigen
Gene Name URL
CD81
Nucleotide Accession Number
NM_133655
Frequently Asked Questions
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