SFTPC ELISA Kit (Rat)
Product Specifications
CAS Number
7732-18-5
Gene Name
Pulmonary surfactant-associated protein C
Gene Aliases
SFTP2
Gene ID
100009162
Accession Number
NP_001075791.1
Reactivity
Rat
Target
Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology SFTPC ELISA Kit (Rat) (OKCA01925) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for SFTPC has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for SFTPC is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample SFTPC captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
4.69 - 300 pg/mL
Recovery
Sample Mean Recovery (%) Range (%) Serum (n=5) 98 94-102 EDTA Plasma (n=4) 87 83-91 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 98 94-102 Serum (n=5) 98 94-102 EDTA Plasma (n=4) 87 83-91 EDTA Plasma (n=4) 87 83-91
Sensitivity
1.17 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
SFTPC
Protein Name
Pulmonary surfactant-associated protein C
Gene Name URL
SFTPC
Nucleotide Accession Number
NM_001082322.1
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