What is the catalog number for PPY ELISA Kit (Rat)?

The catalog number for PPY ELISA Kit (Rat) is OKCA01914-96W.

What sizes are available for PPY ELISA Kit (Rat)?

PPY ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is PPY ELISA Kit (Rat) in stock?

PPY ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

What expression system was used for this recombinant protein?

The expression system, such as E. coli, HEK293, or insect cells, is listed on the datasheet under production details, which can affect post-translational modifications relevant to your application.

Can I return this product if I ordered the wrong one?

As a consumer, you have a 14-day right of withdrawal from receipt under EU consumer protection law, submitted via our withdrawal/cancellation request form. Note this applies to unused, undamaged products, and doesn’t override the expiry/storage conditions on performance-based claims.

Is this product tested for endotoxin levels?

Endotoxin testing status is noted on the datasheet where applicable, particularly for recombinant proteins intended for cell-based or functional assays. If this isn’t listed and you need confirmation, contact support with the catalogue number.

PPY ELISA Kit (Rat)

Selected Variant

OKCA01914-96W

Size: 96 Well

Product Specifications

CAS Number

7732-18-5

Gene Name

Pancreatic hormone

Gene Aliases

PNP, pancreatic polypeptide Y

Accession Number

XP_002719475.3

Reactivity

Rat

Target

Pancreatic hormone is synthesized in pancreatic islets of Langerhans and acts as a regulator of pancreatic and gastrointestinal functions.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology PPY ELISA Kit (Rat) (OKCA01914) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for PPY has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PPY is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PPY captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Lysates

Detection Range

0.78 - 50 pg/mL

Recovery

Sample Mean Recovery (%) Range (%) Serum (n=5) 90 83-94 EDTA Plasma (n=4) 100 91-104 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 90 83-94 Serum (n=5) 90 83-94 EDTA Plasma (n=4) 100 91-104 EDTA Plasma (n=4) 100 91-104

Sensitivity

0.2 pg/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

PPY

Protein Name

Pancreatic hormone

Gene Name URL

PPY

Nucleotide Accession Number

XM_002719429.3

Price

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