What is the catalog number for DEFA4 ELISA Kit (Mouse)?

The catalog number for DEFA4 ELISA Kit (Mouse) is OKCA01733-96W.

What sizes are available for DEFA4 ELISA Kit (Mouse)?

DEFA4 ELISA Kit (Mouse) is available in the following size(s): 96 Well.

Is DEFA4 ELISA Kit (Mouse) in stock?

DEFA4 ELISA Kit (Mouse) is currently made to order / not held in immediate stock. Contact us for lead time.

DEFA4 ELISA Kit (Mouse)

Product Specifications

CAS Number

7732-18-5

Gene Name

Defensin, alpha, 4

Gene Aliases

Cr, Crp4, Defcr, Defa28, Defcr4

Gene ID

13238

Accession Number

NP_034169.2

Reactivity

Mouse

Target

Probably contributes to the antimicrobial barrier function of the small bowel mucosa.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Defa4 ELISA Kit (Mouse) (OKCA01733) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Defa4 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Defa4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Defa4 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Lysates

Detection Range

0.312 - 20 ng/mL

Recovery

Sample Mean Recovery (%) Range (%) Serum (n=5) 99 95-104 EDTA Plasma (n=4) 83 80-86 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 99 95-104 Serum (n=5) 99 95-104 EDTA Plasma (n=4) 83 80-86 EDTA Plasma (n=4) 83 80-86