HBB ELISA Kit (Monkey)
Product Specifications
Gene Name
Hemoglobin subunit beta
Gene Aliases
CD113t-C, beta-globin, beta globin|beta globin chain|hemoglobin beta chain
Accession Number
NP_001157900.1
Reactivity
Monkey
Target
Involved in oxygen transport from the lung to the various peripheral tissues.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology HBB ELISA Kit (Monkey) (OKCA01609) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HBB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HBB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample HBB captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Lysate For Rbc
Detection Range
0.12 - 500 ng/mL
Sensitivity
0.12 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
HBB
Protein Name
Hemoglobin subunit beta
Gene Name URL
HBB
CAS Number
7732-18-5
Nucleotide Accession Number
NM_001164428.1
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