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HBB ELISA Kit (Monkey)

Product Specifications

Gene Name

Hemoglobin subunit beta

Gene Aliases

CD113t-C, beta-globin, beta globin|beta globin chain|hemoglobin beta chain

Accession Number

NP_001157900.1

Reactivity

Monkey

Target

Involved in oxygen transport from the lung to the various peripheral tissues.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology HBB ELISA Kit (Monkey) (OKCA01609) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for HBB has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for HBB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample HBB captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Lysate For Rbc

Detection Range

0.12 - 500 ng/mL

Sensitivity

0.12 ng/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

HBB

Protein Name

Hemoglobin subunit beta

Gene Name URL

HBB

CAS Number

7732-18-5

Nucleotide Accession Number

NM_001164428.1

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