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WFIKKN2 ELISA Kit (Human)

Product Specifications

CAS Number

7732-18-5

Gene Name

WAP, follistatin/kazal, immunoglobulin, kunitz and netrin domain containing 2

Gene Aliases

GASP-1, WFDC20B, hGASP-1, WFIKKNRP

Gene ID

124857

Accession Number

NP_001317270.1

Reactivity

Human

Target

Protease-inhibitor that contains multiple distinct protease inhibitor domains. Probably has serine protease- and metalloprotease-inhibitor activity. Inhibits the biological activity of mature myostatin, but not activin.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology WFIKKN2 ELISA Kit (Human) (OKCA01591) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for WFIKKN2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for WFIKKN2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample WFIKKN2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.156 - 10 ng/mL

Recovery

Sample Mean Recovery (%) Range (%) Serum (n=5) 84 80-89 EDTA Plasma (n=4) 95 91-100 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 84 80-89 Serum (n=5) 84 80-89 EDTA Plasma (n=4) 95 91-100 EDTA Plasma (n=4) 95 91-100

Sensitivity

0.039 ng/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

WFIKKN2

Protein Name

WAP, Kazal, immunoglobulin, Kunitz and NTR domain-containing protein 2

Gene Name URL

WFIKKN2

Nucleotide Accession Number

NM_001330341.1

Frequently Asked Questions

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