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MXRA8 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

Matrix remodeling associated 8

Gene Aliases

ASP3

Gene ID

54587

Accession Number

NP_001269511.1

Reactivity

Human

Target

May play a role in the maturation and maintenance of blood-brain barrier.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology MXRA8 ELISA Kit (Human) (OKCA01352) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MXRA8 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MXRA8 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MXRA8 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

0.156 - 10 ng/mL

Recovery

Sample Mean Recovery (%) Range (%) Serum (n=5) 91 82-94 EDTA Plasma (n=4) 103 94-106 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 91 82-94 Serum (n=5) 91 82-94 EDTA Plasma (n=4) 103 94-106 EDTA Plasma (n=4) 103 94-106

Sensitivity

0.039 ng/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

MXRA8

Protein Name

Matrix-remodeling-associated protein 8

Gene Name URL

MXRA8

Nucleotide Accession Number

NM_001282582.1

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