GPC5 ELISA Kit (Human)
Product Specifications
Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Glypican 5
Gene Aliases
BA93M14.1|glypican proteoglycan 5
Gene ID
2262
Accession Number
NP_004457.1
Reactivity
Human
Target
Cell surface proteoglycan that bears heparan sulfate.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology GPC5 ELISA Kit (Human) (OKCA01260) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GPC5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GPC5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample GPC5 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Ascitic Fluid
Detection Range
0.312 - 20 ng/mL
Recovery
Sample Mean Recovery (%) Range (%) Serum (n=5) 102 95-106 EDTA Plasma (n=4) 95 89-104 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 102 95-106 Serum (n=5) 102 95-106 EDTA Plasma (n=4) 95 89-104 EDTA Plasma (n=4) 95 89-104
Sensitivity
0.078 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
GPC5
Protein Name
Glypican-5
Gene Name URL
GPC5
Nucleotide Accession Number
NM_004466.5
Frequently Asked Questions
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