What is the catalog number for GPC5 ELISA Kit (Human)?

The catalog number for GPC5 ELISA Kit (Human) is OKCA01260-96W.

What sizes are available for GPC5 ELISA Kit (Human)?

GPC5 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is GPC5 ELISA Kit (Human) in stock?

GPC5 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

GPC5 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

Glypican 5

Gene Aliases

BA93M14.1|glypican proteoglycan 5

Gene ID

2262

Accession Number

NP_004457.1

Reactivity

Human

Target

Cell surface proteoglycan that bears heparan sulfate.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology GPC5 ELISA Kit (Human) (OKCA01260) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for GPC5 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for GPC5 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample GPC5 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Ascitic Fluid

Detection Range

0.312 - 20 ng/mL

Recovery

Sample Mean Recovery (%) Range (%) Serum (n=5) 102 95-106 EDTA Plasma (n=4) 95 89-104 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 102 95-106 Serum (n=5) 102 95-106 EDTA Plasma (n=4) 95 89-104 EDTA Plasma (n=4) 95 89-104