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ENPP3 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

Ectonucleotide pyrophosphatase/phosphodiesterase 3

Gene Aliases

B10, NPP3, PDNP3, CD203c, PD-IBETA

Gene ID

5169

Accession Number

NP_005012.2

Reactivity

Human

Target

Cleaves a variety of phosphodiester and phosphosulfate bonds including deoxynucleotides, nucleotide sugars, and NAD.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology ENPP3 ELISA Kit (Human) (OKCA01217) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ENPP3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ENPP3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ENPP3 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Lysates And Tissue Homogenates

Detection Range

0.156 - 10 ng/mL

Recovery

Sample Mean Recovery (%) Range (%) Serum (n=5) 104 98-109 EDTA Plasma (n=4) 85 80-90 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 104 98-109 Serum (n=5) 104 98-109 EDTA Plasma (n=4) 85 80-90 EDTA Plasma (n=4) 85 80-90

Sensitivity

0.039 ng/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

ENPP3

Protein Name

Ectonucleotide pyrophosphatase/phosphodiesterase family member 3

Gene Name URL

ENPP3

Nucleotide Accession Number

NM_005021.4

Frequently Asked Questions

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