CYTL1 ELISA Kit (Human)
Product Specifications
Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Cytokine like 1
Gene Aliases
C17, C4orf4
Gene ID
54360
Accession Number
NP_061129.1
Reactivity
Human
Target
C17 is a cytokine-like protein specifically expressed in bone marrow and cord blood mononuclear cells that bear the CD34 (MIM 142230) surface marker (Liu et al., 2000 [PubMed 10857752]) .[supplied by OMIM, Mar 2008]
Type
ELISA Kit
Assay Principle
Aviva Systems Biology CYTL1 ELISA Kit (Human) (OKCA01190) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CYTL1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CYTL1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CYTL1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.156 - 10 ng/mL
Recovery
Sample Mean Recovery (%) Range (%) Serum (n=5) 95 91-99 EDTA Plasma (n=4) 103 98-108 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 95 91-99 Serum (n=5) 95 91-99 EDTA Plasma (n=4) 103 98-108 EDTA Plasma (n=4) 103 98-108
Sensitivity
0.039 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
CYTL1
Protein Name
Cytokine-like protein 1
Gene Name URL
CYTL1
Nucleotide Accession Number
NM_018659.2
Frequently Asked Questions
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