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CST7 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

Cystatin F

Gene Aliases

CMAP

Gene ID

8530

Accession Number

NP_003641.3

Reactivity

Human

Target

Inhibits papain and cathepsin L but with affinities lower than other cystatins. May play a role in immune regulation through inhibition of a unique target in the hematopoietic system.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology CST7 ELISA Kit (Human) (OKCA01185) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for CST7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CST7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CST7 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Culture Supernates

Detection Range

0.625 - 40 ng/mL

Recovery

Sample Mean Recovery (%) Range (%) Serum (n=5) 100 94-107 EDTA Plasma (n=4) 106 98-112 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 100 94-107 Serum (n=5) 100 94-107 EDTA Plasma (n=4) 106 98-112 EDTA Plasma (n=4) 106 98-112

Sensitivity

0.156 ng/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

CST7

Protein Name

Cystatin-F

Gene Name URL

CST7

Nucleotide Accession Number

NM_003650.3

Frequently Asked Questions

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