ANTXR1 ELISA Kit (Human)
Product Specifications
Reproducibility
Inter-Assay CV:
Gene Name
Anthrax toxin receptor 1
Gene Aliases
ATR, GAPO, TEM8
Gene ID
84168
Accession Number
NP_060623.2
Reactivity
Human
Target
Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology ANTXR1 ELISA Kit (Human) (OKCA01102) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANTXR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANTXR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ANTXR1 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
62.5 - 4,000 pg/mL
Recovery
Sample Mean Recovery (%) Range (%) Serum (n=5) 90 84-95 EDTA Plasma (n=4) 102 95-107 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 90 84-95 Serum (n=5) 90 84-95 EDTA Plasma (n=4) 102 95-107 EDTA Plasma (n=4) 102 95-107
Sensitivity
15.6 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
ANTXR1
Protein Name
Anthrax toxin receptor 1
Gene Name URL
ANTXR1
CAS Number
7732-18-5
Nucleotide Accession Number
NM_018153.3
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