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ANTXR1 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

Anthrax toxin receptor 1

Gene Aliases

ATR, GAPO, TEM8

Gene ID

84168

Accession Number

NP_060623.2

Reactivity

Human

Target

Plays a role in cell attachment and migration. Interacts with extracellular matrix proteins and with the actin cytoskeleton. Mediates adhesion of cells to type 1 collagen and gelatin, reorganization of the actin cytoskeleton and promotes cell spreading. Plays a role in the angiogenic response of cultured umbilical vein endothelial cells.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology ANTXR1 ELISA Kit (Human) (OKCA01102) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ANTXR1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for ANTXR1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample ANTXR1 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

62.5 - 4,000 pg/mL

Recovery

Sample Mean Recovery (%) Range (%) Serum (n=5) 90 84-95 EDTA Plasma (n=4) 102 95-107 Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 90 84-95 Serum (n=5) 90 84-95 EDTA Plasma (n=4) 102 95-107 EDTA Plasma (n=4) 102 95-107

Sensitivity

15.6 pg/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

ANTXR1

Protein Name

Anthrax toxin receptor 1

Gene Name URL

ANTXR1

Nucleotide Accession Number

NM_018153.3

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