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PRL ELISA Kit (Horse)

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

Prolactin

Gene Aliases

GHA1

Gene ID

100034034

Accession Number

NP_001075365.1

Reactivity

Horse

Target

Prolactin acts primarily on the mammary gland by promoting lactation.

Type

ELISA Kit

Assay Principle

This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for PRL and Horseradish Peroxidase (HRP) conjugated PRL. The competitive inhibition reaction is launched between with HRP labeled PRL and unlabeled PRL with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PRL in the sample. The color development is stopped and the intensity of the color is measured.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma

Detection Range

2.5 - 1,000 ng/mL

Recovery

Sample Mean Recovery (%) Range (%) _x005F Serum (n=5) 96 91-105 _x005F EDTA Plasma (n=4) 97 88-102 _x005F Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 96 91-105 Serum (n=5) 96 91-105 EDTA Plasma (n=4) 97 88-102 EDTA Plasma (n=4) 97 88-102

Sensitivity

2.5 ng/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

PRL

Protein Name

Prolactin

Gene Name URL

PRL

Nucleotide Accession Number

NM_001081896.1

Frequently Asked Questions

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