PRL ELISA Kit (Horse)
Product Specifications
Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Prolactin
Gene Aliases
GHA1
Gene ID
100034034
Accession Number
NP_001075365.1
Reactivity
Horse
Target
Prolactin acts primarily on the mammary gland by promoting lactation.
Type
ELISA Kit
Assay Principle
This assay employs the competitive enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for PRL and Horseradish Peroxidase (HRP) conjugated PRL. The competitive inhibition reaction is launched between with HRP labeled PRL and unlabeled PRL with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of PRL in the sample. The color development is stopped and the intensity of the color is measured.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma
Detection Range
2.5 - 1,000 ng/mL
Recovery
Sample Mean Recovery (%) Range (%) _x005F Serum (n=5) 96 91-105 _x005F EDTA Plasma (n=4) 97 88-102 _x005F Sample Mean Recovery (%) Range (%) Sample Mean Recovery (%) Range (%) Serum (n=5) 96 91-105 Serum (n=5) 96 91-105 EDTA Plasma (n=4) 97 88-102 EDTA Plasma (n=4) 97 88-102
Sensitivity
2.5 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
PRL
Protein Name
Prolactin
Gene Name URL
PRL
Nucleotide Accession Number
NM_001081896.1
Frequently Asked Questions
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