IFNG ELISA Kit (Sheep)
Product Specifications
Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Interferon gamma
Gene Aliases
IFN-gamma
Gene ID
443396
Accession Number
NP_001009803.1
Reactivity
Sheep
Target
Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology IFNG ELISA Kit (Sheep) (OKCA01021) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for IFNG has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IFNG is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IFNG captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
5 pg/mL-200 pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 92 87-95 EDTA Plasma (n=4) 86 83-89 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 92 87-95 Serum (n=5) 92 87-95 EDTA Plasma (n=4) 86 83-89 EDTA Plasma (n=4) 86 83-89
Sensitivity
2.5 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
IFNG
Protein Name
Interferon gamma
Gene Name URL
IFNG
Nucleotide Accession Number
NM_001009803.1
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog