What is the catalog number for BGLAP ELISA Kit (Rat)?

The catalog number for BGLAP ELISA Kit (Rat) is OKCA00988-96W.

What sizes are available for BGLAP ELISA Kit (Rat)?

BGLAP ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is BGLAP ELISA Kit (Rat) in stock?

BGLAP ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

BGLAP ELISA Kit (Rat)

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

Osteocalcin

Accession Number

XP_002715429.1

Reactivity

Rat

Target

Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology BGLAP ELISA Kit (Rat) (OKCA00988) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for BGLAP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BGLAP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample BGLAP captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Culture Supernatant, Tissue Homogenates, Urine

Detection Range

0.312 pg/mL-20 pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 86 83-90 EDTA Plasma (n=4) 104 99-109 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 86 83-90 Serum (n=5) 86 83-90 EDTA Plasma (n=4) 104 99-109 EDTA Plasma (n=4) 104 99-109