BGLAP ELISA Kit (Rat)
Product Specifications
Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Osteocalcin
Accession Number
XP_002715429.1
Reactivity
Rat
Target
Constitutes 1-2% of the total bone protein. It binds strongly to apatite and calcium.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology BGLAP ELISA Kit (Rat) (OKCA00988) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for BGLAP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BGLAP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample BGLAP captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Culture Supernatant, Tissue Homogenates, Urine
Detection Range
0.312 pg/mL-20 pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 86 83-90 EDTA Plasma (n=4) 104 99-109 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 86 83-90 Serum (n=5) 86 83-90 EDTA Plasma (n=4) 104 99-109 EDTA Plasma (n=4) 104 99-109
Sensitivity
0.078 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
BGLAP
Protein Name
Osteocalcin
Gene Name URL
BGLAP
Nucleotide Accession Number
XM_002715383.3
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog
MBS2082153-01 0.1 mL
Cy3-Linked Polyclonal Antibody to Non Metastatic Cells 3, Protein NM23A Expressed In (NME3)
Sign In for Pricing
View Details MBS2082153-02 0.2 mL
Cy3-Linked Polyclonal Antibody to Non Metastatic Cells 3, Protein NM23A Expressed In (NME3)
Sign In for Pricing
View Details MBS2082153-03 0.5 mL
Cy3-Linked Polyclonal Antibody to Non Metastatic Cells 3, Protein NM23A Expressed In (NME3)
Sign In for Pricing
View Details MBS2082153-04 1 mL
Cy3-Linked Polyclonal Antibody to Non Metastatic Cells 3, Protein NM23A Expressed In (NME3)
Sign In for Pricing
View Details MBS2082153-05 5 mL
Cy3-Linked Polyclonal Antibody to Non Metastatic Cells 3, Protein NM23A Expressed In (NME3)
Sign In for Pricing
View Details MBS2082153-06 5x 5 mL
Cy3-Linked Polyclonal Antibody to Non Metastatic Cells 3, Protein NM23A Expressed In (NME3)
Sign In for Pricing
View Details