What is the catalog number for PPIA ELISA Kit (Rat)?

The catalog number for PPIA ELISA Kit (Rat) is OKCA00964-96W.

What sizes are available for PPIA ELISA Kit (Rat)?

PPIA ELISA Kit (Rat) is available in the following size(s): 96 Well.

Is PPIA ELISA Kit (Rat) in stock?

PPIA ELISA Kit (Rat) is currently made to order / not held in immediate stock. Contact us for lead time.

PPIA ELISA Kit (Rat)

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

Peptidyl-prolyl cis-trans isomerase A

Gene Aliases

Cyclophilin 18, Cyclophilin A, Cyclosporin A-binding protein, Peptidyl-prolyl cis-trans isomerase A, PPIase A, Rotamase A

Gene ID

100008724

Accession Number

NP_001075526.1

Reactivity

Rat

Target

PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology PPIA ELISA Kit (Rat) (OKCA00964) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for PPIA has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for PPIA is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample PPIA captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Lysates, Tissue Homogenates

Detection Range

125 pg/mL-8,000 pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 106 103-109 EDTA Plasma (n=4) 92 86-98 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 106 103-109 Serum (n=5) 106 103-109 EDTA Plasma (n=4) 92 86-98 EDTA Plasma (n=4) 92 86-98