OSCAR ELISA Kit (Mouse)
Selected Variant
OKCA00910-96W
Size: 96 Well
Product Specifications
Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Osteoclast associated receptor
Gene Aliases
MO, mOSCAR, mOSCAR-M1, mOSCAR-M2, mOSCAR-M3
Gene ID
232790
Accession Number
NP_001277306.1
Reactivity
Mouse
Target
Regulator of osteoclastogenesis which plays an important bone-specific function in osteoclast differentiation.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Oscar ELISA Kit (Mouse) (OKCA00910) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Oscar has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Oscar is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Oscar captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
37.5 pg/mL-2,400 pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 94 89-97 EDTA Plasma (n=4) 91 85-94 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 94 89-97 Serum (n=5) 94 89-97 EDTA Plasma (n=4) 91 85-94 EDTA Plasma (n=4) 91 85-94
Sensitivity
9.38 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
OSCAR
Protein Name
Osteoclast-associated immunoglobulin-like receptor
Gene Name URL
OSCAR
Nucleotide Accession Number
NM_001290377.1
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