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OSCAR ELISA Kit (Mouse)

Selected Variant

OKCA00910-96W

Size: 96 Well

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

Osteoclast associated receptor

Gene Aliases

MO, mOSCAR, mOSCAR-M1, mOSCAR-M2, mOSCAR-M3

Gene ID

232790

Accession Number

NP_001277306.1

Reactivity

Mouse

Target

Regulator of osteoclastogenesis which plays an important bone-specific function in osteoclast differentiation.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Oscar ELISA Kit (Mouse) (OKCA00910) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Oscar has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Oscar is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Oscar captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

37.5 pg/mL-2,400 pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 94 89-97 EDTA Plasma (n=4) 91 85-94 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 94 89-97 Serum (n=5) 94 89-97 EDTA Plasma (n=4) 91 85-94 EDTA Plasma (n=4) 91 85-94

Sensitivity

9.38 pg/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

OSCAR

Protein Name

Osteoclast-associated immunoglobulin-like receptor

Gene Name URL

OSCAR

Nucleotide Accession Number

NM_001290377.1

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