C9ORF72 ELISA Kit (Human)
Product Specifications
Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Chromosome 9 open reading frame 72
Gene Aliases
ALSFTD, DENND9, FTDALS, DENNL72, FTDALS1
Gene ID
203228
Accession Number
NP_001242983.1
Reactivity
Human
Target
May play a role in endosomal trafficking and autophagy.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology C9orf72 ELISA Kit (Human) (OKCA00853) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for C9orf72 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C9orf72 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample C9orf72 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates
Detection Range
31.25 - 2,000 pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 86 83-89 EDTA Plasma (n=4) 91 89-95 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 86 83-89 Serum (n=5) 86 83-89 EDTA Plasma (n=4) 91 89-95 EDTA Plasma (n=4) 91 89-95
Sensitivity
< 7.81 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
C9ORF72
Protein Name
Protein C9orf72
Gene Name URL
C9ORF72
Nucleotide Accession Number
NM_001256054.2
Frequently Asked Questions
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