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C9ORF72 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

Chromosome 9 open reading frame 72

Gene Aliases

ALSFTD, DENND9, FTDALS, DENNL72, FTDALS1

Gene ID

203228

Accession Number

NP_001242983.1

Reactivity

Human

Target

May play a role in endosomal trafficking and autophagy.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology C9orf72 ELISA Kit (Human) (OKCA00853) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for C9orf72 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C9orf72 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample C9orf72 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Lysates

Detection Range

31.25 - 2,000 pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 86 83-89 EDTA Plasma (n=4) 91 89-95 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 86 83-89 Serum (n=5) 86 83-89 EDTA Plasma (n=4) 91 89-95 EDTA Plasma (n=4) 91 89-95

Sensitivity

< 7.81 pg/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

C9ORF72

Protein Name

Protein C9orf72

Gene Name URL

C9ORF72

Nucleotide Accession Number

NM_001256054.2

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