CD247 ELISA Kit (Human)
Product Specifications
Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
CD247 molecule
Gene Aliases
T3Z, CD3H, CD3Q, CD3Z, TCRZ, IMD25, CD3-ZETA
Gene ID
919
Accession Number
NP_000725.1
Reactivity
Human
Target
Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology CD247 ELISA Kit (Human) (OKCA00832) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for CD247 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD247 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CD247 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates
Detection Range
0.156 ng/mL-10 ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 98 93-102 EDTA Plasma (n=4) 93 89-97 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 98 93-102 Serum (n=5) 98 93-102 EDTA Plasma (n=4) 93 89-97 EDTA Plasma (n=4) 93 89-97
Sensitivity
0.039 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
CD247
Protein Name
T-cell surface glycoprotein CD3 zeta chain
Gene Name URL
CD247
Nucleotide Accession Number
NM_000734.3
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