What is the catalog number for CD247 ELISA Kit (Human)?

The catalog number for CD247 ELISA Kit (Human) is OKCA00832-96W.

What sizes are available for CD247 ELISA Kit (Human)?

CD247 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is CD247 ELISA Kit (Human) in stock?

CD247 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

CD247 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

CD247 molecule

Gene Aliases

T3Z, CD3H, CD3Q, CD3Z, TCRZ, IMD25, CD3-ZETA

Gene ID

919

Accession Number

NP_000725.1

Reactivity

Human

Target

Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology CD247 ELISA Kit (Human) (OKCA00832) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for CD247 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD247 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample CD247 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Lysates

Detection Range

0.156 ng/mL-10 ng/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 98 93-102 EDTA Plasma (n=4) 93 89-97 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 98 93-102 Serum (n=5) 98 93-102 EDTA Plasma (n=4) 93 89-97 EDTA Plasma (n=4) 93 89-97