SDC4 ELISA Kit (Human)
Product Specifications
Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Syndecan 4
Gene Aliases
S, S4, Syn, ryud, Synd4, syndec, AA959608, AW108331, ryudocan, syndecan-4
Gene ID
20971
Accession Number
NP_035651.1
Reactivity
Human
Target
Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology Sdc4 ELISA Kit (Human) (OKCA00831) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Sdc4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Sdc4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Sdc4 captured in well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Cell Lysates, Tissue Homogenates
Detection Range
0.625 ng/mL-40 ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 95 91-99 EDTA Plasma (n=4) 94 88-100 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95 91-99 Serum (n=5) 95 91-99 EDTA Plasma (n=4) 94 88-100 EDTA Plasma (n=4) 94 88-100
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
SDC4
Protein Name
Syndecan-4
Gene Name URL
SDC4
Nucleotide Accession Number
NM_011521.2
Frequently Asked Questions
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