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SDC4 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

Syndecan 4

Gene Aliases

S, S4, Syn, ryud, Synd4, syndec, AA959608, AW108331, ryudocan, syndecan-4

Gene ID

20971

Accession Number

NP_035651.1

Reactivity

Human

Target

Cell surface proteoglycan that bears heparan sulfate. Regulates exosome biogenesis in concert with SDCBP and PDCD6IP.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology Sdc4 ELISA Kit (Human) (OKCA00831) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for Sdc4 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Sdc4 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Sdc4 captured in well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Cell Lysates, Tissue Homogenates

Detection Range

0.625 ng/mL-40 ng/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 95 91-99 EDTA Plasma (n=4) 94 88-100 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95 91-99 Serum (n=5) 95 91-99 EDTA Plasma (n=4) 94 88-100 EDTA Plasma (n=4) 94 88-100

Sensitivity

0.156 ng/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

SDC4

Protein Name

Syndecan-4

Gene Name URL

SDC4

Nucleotide Accession Number

NM_011521.2

Frequently Asked Questions

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