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DEK ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

DEK proto-oncogene

Gene Aliases

D6S231E

Gene ID

7913

Accession Number

NP_001128181.1

Reactivity

Human

Target

Involved in chromatin organization.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology DEK ELISA Kit (Human) (OKCA00772) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for DEK has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for DEK is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample DEK captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Lysates

Detection Range

23.44 pg/mL-1,500 pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 95 90-100 EDTA Plasma (n=4) 88 84-92 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 95 90-100 Serum (n=5) 95 90-100 EDTA Plasma (n=4) 88 84-92 EDTA Plasma (n=4) 88 84-92

Sensitivity

5.86 pg/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

DEK

Protein Name

Protein DEK

Gene Name URL

DEK

Nucleotide Accession Number

NM_001134709.1

Frequently Asked Questions

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