MYCN ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

V-myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog

Gene Aliases

NMYC, ODED, MODED, N-myc, bHLHe37

Gene ID

4613

Accession Number

NP_001280157.1

Reactivity

Human

Target

Positively regulates the transcription of NCYM in neuroblastoma cells .

Type

ELISA Kit

Assay Principle

Aviva Systems Biology MYCN ELISA Kit (Human) (OKCA00740) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for MYCN has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for MYCN is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample MYCN captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates, Cell Lysates

Detection Range

15.6 pg/mL-1,000 pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 99 94-101 EDTA Plasma (n=4) 86 82-92 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 99 94-101 Serum (n=5) 99 94-101 EDTA Plasma (n=4) 86 82-92 EDTA Plasma (n=4) 86 82-92