C4ORF48 ELISA Kit (Human)
Product Specifications
Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Neuropeptide-like protein C4orf48
Gene Aliases
Chr4_55, Uncharacterized protein C4orf48
Accession Number
NP_001135408.2
Reactivity
Human
Type
ELISA Kit
Assay Principle
Aviva Systems Biology C4orf48 ELISA Kit (Human) (OKCA00735) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for C4orf48 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for C4orf48 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample C4orf48 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
0.625 ng/mL-40 ng/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 86 81-92 EDTA Plasma (n=4) 102 97-107 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 86 81-92 Serum (n=5) 86 81-92 EDTA Plasma (n=4) 102 97-107 EDTA Plasma (n=4) 102 97-107
Sensitivity
0.156 ng/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
C4ORF48
Protein Name
Neuropeptide-like protein C4orf48
Gene Name URL
C4ORF48
Nucleotide Accession Number
NM_001141936.2
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog