IRF7 ELISA Kit (Human)
Product Specifications
CAS Number
7732-18-5
Gene Name
Interferon regulatory factor 7
Gene Aliases
IMD39, IRF-7, IRF7A, IRF7B, IRF7C, IRF7H, IRF-7H
Gene ID
3665
Accession Number
NP_001563.2
Reactivity
Human
Target
IRF7 encodes interferon regulatory factor 7, a member of the interferon regulatory transcription factor (IRF) family. IRF7 has been shown to play a role in the transcriptional activation of virus-inducible cellular genes, including interferon beta chain genes. Inducible expression of IRF7 is largely restricted to lymphoid tissue. Multiple IRF7 transcript variants have been identified, although the functional consequences of these have not yet been established.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology IRF7 ELISA Kit (Human) (OKCA00703) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IRF7 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IRF7 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IRF7 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates, Cell Lysates
Detection Range
23.4 - 1,500 pg/mL
Recovery
_x005F Matrix Recovery range (%) Average (%) _x005F Serum (n=5) 93 87-97 _x005F EDTA Plasma (n=4) 94 88-97 _x005F Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 93 87-97 Serum (n=5) 93 87-97 EDTA Plasma (n=4) 94 88-97 EDTA Plasma (n=4) 94 88-97
Sensitivity
< 5.8 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
IRF7
Protein Name
Interferon regulatory factor 7
Gene Name URL
IRF7
Nucleotide Accession Number
NM_001572.3
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog