What is the catalog number for IFI27 ELISA Kit (Human)?

The catalog number for IFI27 ELISA Kit (Human) is OKCA00702-96W.

What sizes are available for IFI27 ELISA Kit (Human)?

IFI27 ELISA Kit (Human) is available in the following size(s): 96 Well.

Is IFI27 ELISA Kit (Human) in stock?

IFI27 ELISA Kit (Human) is currently made to order / not held in immediate stock. Contact us for lead time.

IFI27 ELISA Kit (Human)

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

Interferon alpha inducible protein 27

Gene Aliases

P27, ISG12, FAM14D, ISG12A

Gene ID

3429

Accession Number

NP_001123552.1

Reactivity

Human

Target

Promotes cell death. Mediates IFN-induced apoptosis characterized by a rapid and robust release of cytochrome C from the mitochondria and activation of BAX and caspases 2, 3, 6, 8 and 9.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology IFI27 ELISA Kit (Human) (OKCA00702) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IFI27 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IFI27 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IFI27 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

28 pg/mL-1,800 pg/mL

Recovery

Matrix Recovery range (%) Average (%) Serum (n=5) 87 84-90 EDTA Plasma (n=4) 99 95-102 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 87 84-90 Serum (n=5) 87 84-90 EDTA Plasma (n=4) 99 95-102 EDTA Plasma (n=4) 99 95-102