COLEC10 ELISA Kit (Human)
Product Specifications
Reproducibility
Inter-Assay CV:
CAS Number
7732-18-5
Gene Name
Collectin subfamily member 10
Gene Aliases
3MC3, CLL1, CL-34
Gene ID
10584
Accession Number
NP_001311024.1
Reactivity
Human
Target
Lectin that binds to various sugars: galactose > mannose = fucose > N-acetylglucosamine > N-acetylgalactosamine.
Type
ELISA Kit
Assay Principle
Aviva Systems Biology COLEC10 ELISA Kit (Human) (OKCA00641) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for COLEC10 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for COLEC10 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample COLEC10 captured in the well.
Assay Protocol
Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions
Reconstitution & Storage Instructions
Western Blotting/Immunoblotting (WB/IB) Protocol
Western Blotting/Immunoblotting (WB/IB) Protocol
Immunohistochemistry (IHC) Protocol
Immunohistochemistry (IHC) Protocol
Immunocytochemistry (ICC) Protocol
Immunocytochemistry (ICC) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
Blocking Peptide Competition Protocol (BPCP)
Blocking Peptide Competition Protocol (BPCP)
Immunoprecipitation (IP) Protocol
Immunoprecipitation (IP) Protocol
Antibody Array (AA) Protocol
Antibody Array (AA) Protocol
Sample Type
Serum, Plasma, Tissue Homogenates
Detection Range
23.44 pg/mL-1,500 pg/mL
Recovery
Matrix Recovery range (%) Average (%) Serum (n=5) 96 92-102 EDTA Plasma (n=4) 88 82-94 Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 96 92-102 Serum (n=5) 96 92-102 EDTA Plasma (n=4) 88 82-94 EDTA Plasma (n=4) 88 82-94
Sensitivity
5.86 pg/mL
Reconstitution
Store as indicated in product manual.
NCBI Gene Symbol
COLEC10
Protein Name
Collectin-10
Gene Name URL
COLEC10
Nucleotide Accession Number
NM_001324095.1
Frequently Asked Questions
More Discoveries
Explore Other Products
Browse additional items from our catalog