What is the catalog number for IL2 ELISA Kit (Goat)?

The catalog number for IL2 ELISA Kit (Goat) is OKCA00589-96W.

What sizes are available for IL2 ELISA Kit (Goat)?

IL2 ELISA Kit (Goat) is available in the following size(s): 96 Well.

Is IL2 ELISA Kit (Goat) in stock?

IL2 ELISA Kit (Goat) is currently made to order / not held in immediate stock. Contact us for lead time.

Do you offer custom or private-label production of this type of product?

Yes, custom antibody development and private-label production services are available. Contact our sales team to discuss your target, timeline, and quantity requirements.

What is the immunogen used to generate this antibody?

Immunogen or antigen details used to raise this antibody are listed on the product datasheet under immunogen information.

What is the recommended positive control for this assay?

A recommended positive control, where one is specified by the manufacturer, is listed on the datasheet or in the assay protocol.

IL2 ELISA Kit (Goat)

Product Specifications

Reproducibility

Inter-Assay CV:

CAS Number

7732-18-5

Gene Name

Interleukin-2

Accession Number

NP_001274496.1

Reactivity

Goat

Target

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.

Type

ELISA Kit

Assay Principle

Aviva Systems Biology IL2 ELISA Kit (Goat) (OKCA00589) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for IL2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) . Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for IL2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample IL2 captured in the well.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Serum, Plasma, Tissue Homogenates

Detection Range

15.6 pg/mL-1,000 pg/mL

Recovery

_x005F Matrix Recovery range (%) Average (%) _x005F Serum (n=5) 97 95-102 _x005F EDTA Plasma (n=4) 93 88-97 _x005F Matrix Recovery range (%) Average (%) Matrix Recovery range (%) Average (%) Serum (n=5) 97 95-102 Serum (n=5) 97 95-102 EDTA Plasma (n=4) 93 88-97 EDTA Plasma (n=4) 93 88-97

Sensitivity

3.9 pg/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

IL2

Protein Name

Interleukin-2

Gene Name URL

IL2

Nucleotide Accession Number

NM_001287567.1

Frequently Asked Questions

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