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RuBisCO ELISA Kit (Plant)

Product Specifications

Reproducibility

Mean Intra-Assay Precision %CV: < 10% (n = 20) Mean Inter-Assay Precision %CV: < 20% (n = 20)

CAS Number

7732-18-5

Reactivity

Plant

Type

ELISA Kit

Assay Principle

Aviva Systems Biology RuBisCO ELISA Kit (Plant) (OKCA00374) is based on a competitive enzyme immuno assay technique. The microtiter plate provided in this kit has been pre-coated with goat-anti-rabbit antibody. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for RuBisCO and Horseradish Peroxidase (HRP) conjugated RuBisCO. The competitive inhibition reaction is launched between with HRP labeled RuBisCO and unlabeled RuBisCO with the antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of RuBisCO in the sample. The color development is stopped and the intensity of the color is measured.

Assay Protocol

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol

Sample Type

Plant tissues

Detection Range

3.13 ug/mL - 800 ug/mL

Recovery

Sample 1 Sample Type Average Recovery Range leaf tissue (n=5) 0.95 89-103% Sample 1 Sample 1 Sample Type Average Recovery Range Sample Type Average Recovery Range leaf tissue (n=5) 0.95 89-103% leaf tissue (n=5) 0.95 89-103%

Sensitivity

< 3.13 ug/mL

Reconstitution

Store as indicated in product manual.

NCBI Gene Symbol

RuBisCO

Protein Name

RuBisCO

Gene Name URL

RuBisCO

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