Free-Thyroxine 4 ELISA Kit (Fish)

Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 Mean (pmolL 16.075 16.13 16.224 16.163 15.986 16.104 SD 0.039 0.042 0.035 0.052 0.055 0.06 CV% 4.561 4.928 4.108 6.008 6.323 6.947 Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Mean Intra-Assay Precision %CV Mean Inter-Assay Precision %CV Sample 1 2 3 1 2 3 Sample 1 2 3 1 2 3 n 20 20 20 20 20 20 n 20 20 20 20 20 20 Mean (pmolL 16.075 16.13 16.224 16.163 15.986 16.104 Mean (pmolL Mean (pmolL 16.075 16.13 16.224 16.163 15.986 16.104 SD 0.039 0.042 0.035 0.052 0.055 0.06 SD 0.039 0.042 0.035 0.052 0.055 0.06 CV% 4.561 4.928 4.108 6.008 6.323 6.947 CV% 4.561 4.928 4.108 6.008 6.323 6.947

Aviva Systems Biology Free-Thyroxine 4 ELISA Kit (Fish) (OKCA00355) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Fish Free-Thyroxine 4 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Free-Thyroxine 4 and incubated. The Free-Thyroxine 4 found in the sample or standards competes with the biotinylated Free-Thyroxine 4 for limited binding sites on the immobilized anti-Fish Free-Thyroxine 4 antibody. Excess unbound biotinylated Free-Thyroxine 4 and sample or standard Free-Thyroxine 4 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Free-Thyroxine 4 captured in the well and inversely proportional to the amount of Free-Thyroxine 4 which was contained in the sample or standard.

Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Reconstitution & Storage Instructions Reconstitution & Storage Instructions Western Blotting/Immunoblotting (WB/IB) Protocol Western Blotting/Immunoblotting (WB/IB) Protocol Immunohistochemistry (IHC) Protocol Immunohistochemistry (IHC) Protocol Immunocytochemistry (ICC) Protocol Immunocytochemistry (ICC) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol Blocking Peptide Competition Protocol (BPCP) Blocking Peptide Competition Protocol (BPCP) Immunoprecipitation (IP) Protocol Immunoprecipitation (IP) Protocol Antibody Array (AA) Protocol Antibody Array (AA) Protocol